SNP Discovery & Resequencing
For customers interested in high throughput SNP discovery, Cogenics conducts 96- or 384-well amplification and sequencing of multiple plant, animal and human samples with subsequent mutation identification.

As part of the SNP discovery and resequencing service, Cogenics will design and synthesize human custom primers or identify and use appropriate primers from its proprietary HAP® Database. The HAP® Database is a collection of SNPs and haplotypes (which we call HAP™ Markers) for > 8,000 genes. The HAP® Database includes location and context sequence of each SNP discovered in Cogenics’ standard Index Repository (representing the major populations of the world in 93 well-characterized individuals), HAP™ Markers discovered in this Index Repository, and frequencies of SNPs and HAP™ Markers in each population group in the Index Repository. Cogenics’ DecoGen® Informatics System performs data analysis of HAP® Database information, with the ability to identify probable associations between SNPs and clinical outcomes. DecoGen® is also compatible with SNP data from public and client databases.

If clients do not wish to access our HAP® Database, Cogenics will analyze result sequencing data for SNPs using Softgenetics’ Mutation Surveyor software.

Benefits of Cogenics’ SNP discovery & resequencing service include:

• Access to proprietary HAP® Database content
• Custom primer design and synthesis
• Highest quality sequencing data

Samples
Clients submit purified DNA in 96-well plates and primer sequence for plant and animal samples. For human samples, customers need only provide purified DNA in 96-well plates.

Results
All Final Reports include SNP scores and identification. Projects incorporating HAP® Database content also include the genes and SNPs analyzed as well as possible mechanisms underlying observed associations and haplotype marker identification tables.