| SAMPLE TYPE |
MINIMUM SAMPLE REQUIREMENTS |
| Express Sequencing |
Plasmid DNA or Purified PCR Products |
500 ng per sequencing reaction at 50 ng/µl in 10 µl of water |
Plasmid DNA and Primer Mixed |
500 ng per sequencing reaction at 50 ng/µl - DNA 8 pmol Primer at 0.8 pmol/µl - Primer in a total volume of 10 µl |
Primer |
8 pmol per sequencing reaction at 0.8 pmol/µl in 10 µl |
| Sequencing |
Regulatory Submission Sequencing
Bacterial Samples |
Low Copy- 1-2 mls of 109-1010cells/ml
High Copy- 1-2 mls of 108-109 cells/ml |
Regulatory Submission Sequencing
Yeast Samples |
1-2 mls of 108 cells/ml |
Regulatory Submission Sequencing
Mammalian Samples |
1-2 ml of 107 cells/ml |
| |
|
Research Grade Sequencing
Plasmid DNA |
300-500 ng per sequencing reaction (minimum concentration 100ng/µl)
DNA as a dried down pellet, or in molecular biology grade water or elution buffer, NOT in TE. |
Research Grade Sequencing
PCR Product |
20 ng for each 100 bp of amplicon per sequencing reaction (PCR Products must be single band and purified, additional charges will incur for enzymatic purification or gel extraction) |
| |
|
High Throughput Sequencing
(96-well format)
Bacterial Samples |
Glycerol stock-HT (~108 cells/ml) 100 µl/ sample |
High Throughput Sequencing
(96-well format)
DNA |
10µl at 100ng/µl per well |
| |
|
Sequencing-All types
Stab or streak cultures |
1 stab or plate |
| |
|
| Viral DNA |
generally 300 ng of DNA per sequencing reaction (exact amount will be included in quotation) |
| BAC clone |
generally 300 ng of DNA per sequencing reaction (exact amount will be included in quotation) |
| RNA |
2 µg minimum in water, TE or elution buffer |
| |
|
| Primer |
10 µl of 8 pmol/µl dilution of the primer per sequencing reaction
(50 ng/ul for a 20-mer)
Please note that not all PCR primers are optimum for sequencing. Primers should be approximately 20 bases long and the melting temperature should be between 50-65 degrees Celsius. |
| Shotgun Library Construction |
| Purified viral particles |
1-2 mls of 1012 pfu/ml |
| DNA |
20 µg |
| Stab or plates |
1 stab or plate |
| Glycerol Stock |
100 µl (~108 cells/ml) |
| Genetic Stability Testing |
Southern Blot-Copy Number
Southern Blot-Insertion Number
Southern Blot-Structural Map |
This amount will vary depeding on the digest number and the blot set up. Please see your quotation for materials required.
Mammalian = 20 µg of DNA/digest
Yeast and Bacteria 5-10 µg DNA/digest |
Phage Testing
Plasmid Loss |
Bacterial 1ml (108 cells/ml) |
Northern Blot
Restriction Enzyme Analysis |
Bacterial 1ml (108 cells/ml)
Yeast 1 ml (107 cells/ml)
Mammalian 1ml (106 cells/ml)
(wet cell pellet - at least 3 grams or 100 ml) |
| Quantitative PCR |
| Cell Banks - Bacterial |
1-2 ml (108 cells/ml) |
| Cell Banks - Yeast |
1-2 ml (107 cells/ml) |
| Cell Banks - Mammalian |
1-2 ml (106 cells/ml) |
| Tissue |
10-25 mg |
| Whole Blood |
1 ml |
| DNA |
500 ng |
| RNA |
500 ng |
| GMO testing |
| PCR Method |
500 ng of young leaf per sample |
| Southern Method |
3 g of young leaf per sample |
| Genotyping |
| PCR/Agarose Gel method |
50-100 ng per PCR reaction |
| SNP & STR |
custom, please call Cogenics for further information |
| Other Services |
| Subcloning |
custom, please call Cogenics for further information |
Northern Blot
Restriction Enzyme Analysis |
Bacterial 1ml (108 cells/ml)
Yeast 1 ml (107 cells/ml)
Mammalian 1ml (106 cells/ml)
(wet cell pellet - at least 3 grams or 100 ml) |
